製品の概要

  • 製品名Anti-TRIP12 antibody
  • 製品の詳細
    Rabbit polyclonal to TRIP12
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Camel, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 75 and 125 of human TRIP12 (NP_004229.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab86220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 220 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent multisubunit protein that activates the proteolytic activities of the multifunctional proteinase (20S proteasome) of the 26S complex. TRP12 specifically interacts with the ligand binding domain of the thyroid hormone receptor (in a thyroid hormone T3-independent manner) and with retinoid X receptor (RXR). TRP12 was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1.
    • 参照データベース
    • 別名
      • KIAA0045 antibody
      • MGC138849 antibody
      • MGC138850 antibody
      • Thyroid hormone receptor interactor 12 antibody
      • Thyroid receptor interacting protein 12 antibody
      • TRIP 12 antibody
      see all

    Anti-TRIP12 antibody 画像

    • All lanes : Anti-TRIP12 antibody (ab86220) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 1.5 µg
      Lane 3 : HeLa whole cell lysate at 0.5 µg

      Developed using the ECL technique

      Predicted band size : 220 kDa


      Exposure time : 3 minutes
    • Detection of human TRIP12 in Immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded) using ab86220 at 3 µg/mg lysate for IP, and at 0.1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-TRIP12 antibody (ab86220) 使用論文

    ab86220 has not yet been referenced specifically in any publications.

    Product Wall

    We do not have data for mouse reactivity for this TRIP12 antibody, ab86220. The amino acid sequence of the immunogen that generated the antibody is well-conserved in the mouse protein so cross-reaction is possible, though not experimentally confirmed.<...

    Read More

    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"