The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml.
Use a concentration of 2 µg/ml. Predicted molecular weight: 53 kDa. additonal band of unkown identity was also concistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide.
Inhibits activation of NF-kappa-B mediated by TNF.
Anti-TRIP antibody (ab4533) at 2 µg/ml + Human Testis lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa Observed band size: 53 kDa Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry - Anti-TRIP antibody (ab4533)
In paraffin embedded Human Spleen shows perinucxlear and textured cystoplasmic staining in selective areas.ab4533 (4µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.