製品の概要

  • 製品名Anti-TRIM72 antibody
    TRIM72 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRIM72
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Human, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TRIM72.

  • ポジティブ・コントロール
    • WB: Mouse heart, rat heart, mouse skeletal muscle and rat skeletal muscle tissue lysates. ICC/IF: SV40LT-SMC cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB 1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Abcam recommends using BSA as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.

ターゲット情報

  • 機能Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • 配列類似性Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • 翻訳後修飾Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.
  • 細胞内局在Cell membrane > sarcolemma. Cytoplasmic vesicle membrane. Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LOC493829 antibody
    • Mg53 antibody
    • Mitsugumin-53 antibody
    • TRI72_HUMAN antibody
    • trim72 antibody
    • Tripartite motif containing 72 antibody
    • Tripartite motif containing protein 72 antibody
    • Tripartite motif-containing protein 72 antibody
    see all

Anti-TRIM72 antibody 画像

  • All lanes : Anti-TRIM72 antibody (ab118651) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 4 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 24 kDa,42 kDa,49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    Abcam recommends using BSA as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • ICC/IF image of ab118651 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118651, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TRIM72 antibody (ab118651) 使用論文

ab118651 has not yet been referenced specifically in any publications.

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