製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to TRIM71
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids of Human TRIM71.

  • ポジティブ・コントロール
    • Human Brain Cerebellum tissue; HEK293 and HEK293 lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab117607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 93 kDa.
IHC-P Use a concentration of 3.75 µg/ml.

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.
  • 組織特異性
    Specifically expressed in testis.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • 翻訳後修飾
    Autoubiquitinated.
  • 細胞内局在
    Cytoplasm, P-body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2610206G21Rik antibody
    • Abnormal cell LINeage LIN 41 antibody
    • AL022943 antibody
    • E3 ubiquitin-protein ligase TRIM71 antibody
    • Gm1127 antibody
    • Homolog of C. elegans Lin 41 antibody
    • LIN 41 antibody
    • LIN41 antibody
    • LIN41_HUMAN antibody
    • mlin 41 antibody
    • Protein lin 41 homolog antibody
    • Protein lin-41 homolog antibody
    • RGD1566388 antibody
    • Ripply2 antibody
    • TRIM 71 antibody
    • TRIM71 antibody
    • Tripartite motif containing 71 antibody
    • Tripartite motif-containing protein 71 antibody
    see all

Anti-TRIM71 antibody 画像

  • ab117607, at 3.75 µg/ml, staining TRIM71 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry.
  • Anti-TRIM71 antibody (ab117607) at 0.5 µg/ml + HEK293 lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 93 kDa

Anti-TRIM71 antibody (ab117607) 使用論文

ab117607 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (2 day embryos)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (7% gel)
Loading amount
75 µg
Specification
2 day embryos
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Mar 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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