製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRIM68
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (IRLRKGNEYR AGTDEYPILS LPVPPRRVGI FVDYEAHDIS FYNVTDCGSH) of human TRIM68 (NP_060543).

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.
  • 組織特異性
    Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • ドメイン
    The RING domain is essential for ubiquitin E3 ligase activity.
  • 翻訳後修飾
    Auto-ubiquitinated.
  • 細胞内局在
    Cytoplasm > perinuclear region. Nucleus. Colocalized with AR in nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase TRIM68 antibody
    • FLJ10369 antibody
    • GC109 antibody
    • MGC126176 antibody
    • RING finger protein 137 antibody
    • RNF 137 antibody
    • RNF137 antibody
    • Ro/SSA1 related protein antibody
    • SS 56 antibody
    • SS-56 antibody
    • SS56 antibody
    • SSA protein SS 56 antibody
    • SSA protein SS-56 antibody
    • TRI68_HUMAN antibody
    • TRIM 68 antibody
    • Trim68 antibody
    • Tripartite motif containing 68 antibody
    • Tripartite motif containing protein 68 antibody
    • Tripartite motif-containing protein 68 antibody
    see all

画像

  • Anti-TRIM68 antibody (ab86393) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 36 kDa,42 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TRIM68 with ab86393 at 5.0µg/ml.

参考文献

ab86393 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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