製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRIM62
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    A region within synthetic peptide: EHWVRQEAQG ARDCPECRRT FAEPALAPSL KLANIVERYS SFPLDAILNA, corresponding to N terminal amino acids 37-86 of Human TRIM62

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

 Titre using peptide based assay: 1/1562500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • ドメイン
    The RING finger is required for ubiquitin ligase activity.
  • 翻訳後修飾
    Polyubiquitinated, autoubiquitinated in the presence of UBE2D2.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6330414G21Rik antibody
    • AI450348 antibody
    • DEAR1 antibody
    • ductal epithelium-associated RING Chromosome 1 antibody
    • E3 ubiquitin-protein ligase TRIM62 antibody
    • FLJ10759 antibody
    • FLJ16558 antibody
    • MGC115263 antibody
    • TRI62_HUMAN antibody
    • TRIM62 antibody
    • Tripartite motif containing 62 antibody
    • Tripartite motif-containing protein 62 antibody
    see all

画像

  • Anti-TRIM62 antibody (ab51039) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab51039 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Formalin, 8 hours
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Yunhua Bao

Verified customer

投稿 May 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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