製品の概要
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製品名Anti-TRIM5 gamma antibody
TRIM5 gamma 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TRIM5 gamma
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由来種Rabbit
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アプリケーション適用あり: WB, ELISA, IHC-Pmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide conjugated to KLH corresponding to 15 amino acids near the carboxy-terminus of Human TRIM5 gamma.
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ポジティブ･コントロール
- Human Small Intestine and Human Pancreas tissue. Human bladder and colon cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab109763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
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パスウェイProtein modification; protein ubiquitination.
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配列類似性Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
ドメインThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
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翻訳後修飾Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
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細胞内局在Cytoplasm > P-body. Cytoplasmic bodies.
- Information by UniProt
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参照データベース
- Entrez Gene: 85363 Human
- Omim: 608487 Human
- SwissProt: Q9C035 Human
- Unigene: 370515 Human
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別名
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
see all
画像
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Western blot - Anti-TRIM5 gamma antibody (ab109763)All lanes : Anti-TRIM5 gamma antibody (ab109763) at 2 µg/ml
Lane 1 : Human bladder lysate
Lane 2 : Human colon lysate
Predicted band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 gamma antibody (ab109763)ab109763, at 2.5 µg/ml, staining TRIM5 gamma in formalin-fixed, paraffin-embedded Human Small Intestine tissue by immunohistochemistry. Sections were incubated with a biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 gamma antibody (ab109763)ab109763, at 2.5 µg/ml, staining TRIM5 gamma in formalin-fixed, paraffin-embedded Human pancreas tissue by immunohistochemistry. Sections were incubated with a biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
参考文献
ab109763 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"