Anti-TRIM5 alpha 抗体 (ab109709)
Key features and details
- Rabbit polyclonal to TRIM5 alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRIM5 alpha antibody
TRIM5 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRIM5 alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Macaque monkey, Gorilla -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: Human Placenta; Human Thymus; Human Tonsil.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109709の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
ドメイン
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. -
翻訳後修飾
Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro). -
細胞内局在
Cytoplasm > P-body. Cytoplasmic bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 503563 Chimpanzee
- Entrez Gene: 101133340 Gorilla
- Entrez Gene: 85363 Human
- Omim: 608487 Human
- SwissProt: Q5D7J1 Chimpanzee
- SwissProt: Q5C8T6 Gorilla
- SwissProt: Q9C035 Human
- Unigene: 370515 Human
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別名
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
see all
画像
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All lanes : Anti-TRIM5 alpha antibody (ab109709) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Tonsil (Human) Tissue Lysate
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 31 kDa (possible isoform), 46 kDa (possible isoform)
Exposure time: 2 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms Alpha, Beta and Epsilon of TRIM5. The predicted molecular weights of isoforms Alpha, Beta and Epsilon are 56, 46 and 31 kDa respectively. -
IHC image of TRIM5 alpha staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109709, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab109709 は 1 報の論文で使用されています。
- Rebensburg SV et al. Sec24C is an HIV-1 host dependency factor crucial for virus replication. Nat Microbiol 6:435-444 (2021). PubMed: 33649557