製品の概要

  • 製品名
    Anti-TRIM49 antibody
  • 製品の詳細
    Rabbit polyclonal to TRIM49
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (RPCFYLNWQD IPFLVQCSEC TKSTEQINLK TNIHLKKMAS LARKVSLWLF) of human TRIM49 (NP_065091).

  • ポジティブ・コントロール
    • Fetal liver lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 組織特異性
    Preferentially expressed in testis.
  • 配列類似性
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Information by UniProt
  • 参照データベース
  • 別名
    • RING finger protein 18 antibody
    • RNF 18 antibody
    • RNF18 antibody
    • Testis specific RING Finger protein antibody
    • Testis-specific ring-finger protein antibody
    • TRI49_HUMAN antibody
    • TRIM 49 antibody
    • TRIM49 antibody
    • Tripartite motif containing 49 antibody
    • Tripartite motif containing protein 49 antibody
    • Tripartite motif-containing protein 49 antibody
    see all

画像

  • Anti-TRIM49 antibody (ab82779) at 1 µg/ml (5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab82779 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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