The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects bands of approximately 70 and 80 and 130 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
TRIM47 belongs to the TRIM/RBCC family. It contains one B box type zinc finger, one B30.2/SPRY domain and one RING type zinc finger. TRIM47 has low expression in most tissues; higher expression in kidney tubular cells and over expressed in astrocytoma tumor cells.
Gene overexpressed in astrocytoma protein antibody
RING finger protein 100 antibody
Tripartite motif containing 47 antibody
Tripartite motif containing protein 47 antibody
Western blot - TRIM47 antibody (ab72234)
All lanes : Anti-TRIM47 antibody (ab72234) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of TRIM47.
Lane 1: ab72234 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72234 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.