製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRIM45
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide within residues: FKAPRLLPCL HTVCTTCLEQ LEPFSVVDIR GGDSDTSSEG SIFQELKPRS , corresponding to N terminal amino acids 36-85 of Human TRIM45 (NP_079464)

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.
  • 組織特異性
    Expressed in skeletal muscle, brain, heart and pancreas.
  • 配列類似性
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 filamin repeat.
    Contains 1 RING-type zinc finger.
  • 発生段階
    Expressed in brain, lung, skeletal muscle, heart and intestine in 80-day old embryo.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ13181 antibody
    • RING finger protein 99 antibody
    • RNF99 antibody
    • TRI45_HUMAN antibody
    • TRIM45 antibody
    • Tripartite motif containing 45 antibody
    • Tripartite motif containing protein 45 antibody
    • Tripartite motif-containing protein 45 antibody
    see all

画像

  • Anti-TRIM45 antibody (ab84303) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa

プロトコール

参考文献

This product has been referenced in:
  • Sato T  et al. The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway. Oncogene 0:N/A (2014). Read more (PubMed: 24681954) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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