製品の概要

  • 製品名Anti-TRIM29 antibody
    TRIM29 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRIM29
  • アプリケーション適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 538 and 588 of human TRIM29 (NP_036233.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab74193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能It is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
    • 組織特異性Expressed in placenta, prostate and thymus.
    • 配列類似性Contains 1 B box-type zinc finger.
    • 翻訳後修飾Constitutively phosphorylated by PKC on serine/threonine in A431 cells.
    • 細胞内局在Cytoplasm. Colocalizes with intermediate filaments.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Ataxia telangiectasia group D associated protein antibody
      • Ataxia telangiectasia group D-associated protein antibody
      • ATDC antibody
      • FLJ36085 antibody
      • TRI29_HUMAN antibody
      • TRIM 29 antibody
      • TRIM29 antibody
      • Tripartite motif containing 29 antibody
      • Tripartite motif containing protein 29 antibody
      • Tripartite motif protein 29 antibody
      • Tripartite motif protein TRIM29 antibody
      • Tripartite motif-containing protein 29 antibody
      see all

    Anti-TRIM29 antibody 画像

    • Detection of TRIM29 by Western Blot of Immunprecipitate.
      ab74193 at 1µg/ml staining TRIM29 in HeLa whole cell lysate immunoprecipitated using ab74193 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.
    • IHC image of ab74193 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74193, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-TRIM29 antibody (ab74193) 使用論文

    ab74193 has not yet been referenced specifically in any publications.

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