製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to TRIM26
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human TRIM26 (NP_003440.1, amino acids 1-539)

  • ポジティブ・コントロール
    • A-431 cell lysate and TRIM26 transfected 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.

ターゲット情報

  • 配列類似性
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acid finger protein antibody
    • AFP antibody
    • CAM25547 antibody
    • RING finger protein 95 antibody
    • RNF95 antibody
    • TRI26_HUMAN antibody
    • TRIM26 antibody
    • tripartite motif containing 26 antibody
    • Tripartite motif-containing protein 26 antibody
    • widely expressed acid zinc finger protein antibody
    • Zinc finger protein 173 antibody
    • Zinc finger protein 173, formerly antibody
    • ZNF173 antibody
    • ZNF173, formerly antibody
    see all

画像

  • Anti-TRIM26 antibody (ab89290) at 1 µg/ml + A-431 cell lysate at 50 µg

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-TRIM26 antibody (ab89290) at 1 µg/ml

    Lane 1 : TRIM26 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Edmonds MJ  et al. Ubiquitylation-dependent regulation of NEIL1 by Mule and TRIM26 is required for the cellular DNA damage response. Nucleic Acids Res 45:726-738 (2017). Read more (PubMed: 27924031) »
  • Zhang X  et al. Spina bifida in fetus is associated with an altered pattern of DNA methylation in placenta. J Hum Genet N/A:N/A (2015). Read more (PubMed: 26178427) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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