リコンビナント
RabMAb

Anti-TRIM25 抗体 [EPR7315] (ab167154)

製品の概要

  • 製品名
    Anti-TRIM25 antibody [EPR7315]
    TRIM25 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR7315] to TRIM25
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    corresponding to Human TRIM25.

  • ポジティブ・コントロール
    • HeLa and K562 cell lysates; Human breast tissue. Human pancreas tissue. Rat brain lysate. SP2/0 (mouse spleen) whole cell lysate.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab167154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/50000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/500 - 1/1000.
IP 1/40 - 1/100.
Flow Cyt 1/60 - 1/90.

ターゲット情報

  • 機能
    Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
  • 組織特異性
    Ubiquitous.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • ドメイン
    The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
    The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
  • 翻訳後修飾
    Auto-ISGylated.
  • 細胞内局在
    Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin/ISG15 ligase TRIM25 antibody
    • EFP antibody
    • Estrogen responsive finger protein antibody
    • Estrogen-responsive finger protein antibody
    • RING finger protein 147 antibody
    • RNF 147 antibody
    • RNF147 antibody
    • TRI25 antibody
    • TRI25_HUMAN antibody
    • TRIM 25 antibody
    • Trim25 antibody
    • Tripartite motif containing 25 antibody
    • Tripartite motif containing protein 25 antibody
    • Tripartite motif protein TRIM25 antibody
    • Tripartite motif-containing protein 25 antibody
    • Tripartite motifcontaining25 antibody
    • Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody
    • Z147 antibody
    • Zinc finger protein 147 (estrogen responsive finger protein) antibody
    • Zinc finger protein 147 antibody
    • Zinc finger protein-147 antibody
    • ZNF 147 antibody
    • ZNF147 antibody
    see all

画像

  • All lanes : Anti-TRIM25 antibody [EPR7315] (ab167154) at 1/50000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 71 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

  • ab167154 staining TRIM25 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

     

  • ab167154 staining TRIM25 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a tubulin counterstain at a dilution of 1/200 and DAPI was used as a nuclear counterstain.

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling TRIM25 with purified ab167154 at 1/90 dilution(10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Anti-TRIM25 antibody [EPR7315] (ab167154) at 1/2000 dilution + SP2/0 (mouse spleen) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 71 kDa
    Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.

    Blocking and diluting buffer: 5% NFDM /TBST 

  • ab167154 immunoprecipitating TRIM25. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/40 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate (10ug)
    Lane 2: HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab167154 in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate.

  • Anti-TRIM25 antibody [EPR7315] (ab167154) at 1/10000 dilution + Rat brain lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 71 kDa
    Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.

    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-TRIM25 antibody [EPR7315] (ab167154) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 71 kDa
  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TRIM25 with ab167154 at 1/100 dilution.
  • Immunofluorescent analysis of HeLa cells labeling TRIM25 with ab167154 at 1/100 dilution.

参考文献

This product has been referenced in:
  • Shi Y  et al. Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity. Nat Commun 8:15138 (2017). WB ; Human . Read more (PubMed: 28469175) »
  • Lang F  et al. Dynamic changes in the mouse skeletal muscle proteome during denervation-induced atrophy. Dis Model Mech 10:881-896 (2017). WB ; Mouse . Read more (PubMed: 28546288) »

See all 3 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab167154.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録