製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to TRIM25
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    TRIM25 full-length Human protein ((AAH16924.1)

  • ポジティブ・コントロール
    • Hela NE lysate, HeLa cell and TRIM25 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
  • 組織特異性
    Ubiquitous.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • ドメイン
    The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
    The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
  • 翻訳後修飾
    Auto-ISGylated.
  • 細胞内局在
    Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin/ISG15 ligase TRIM25 antibody
    • EFP antibody
    • Estrogen responsive finger protein antibody
    • Estrogen-responsive finger protein antibody
    • RING finger protein 147 antibody
    • RNF 147 antibody
    • RNF147 antibody
    • TRI25 antibody
    • TRI25_HUMAN antibody
    • TRIM 25 antibody
    • Trim25 antibody
    • Tripartite motif containing 25 antibody
    • Tripartite motif containing protein 25 antibody
    • Tripartite motif protein TRIM25 antibody
    • Tripartite motif-containing protein 25 antibody
    • Tripartite motifcontaining25 antibody
    • Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody
    • Z147 antibody
    • Zinc finger protein 147 (estrogen responsive finger protein) antibody
    • Zinc finger protein 147 antibody
    • Zinc finger protein-147 antibody
    • ZNF 147 antibody
    • ZNF147 antibody
    see all

画像

  • Anti-TRIM25 antibody (ab88669) at 1 µg/ml + Hela nuclear extract lysate at 50 µg

    Predicted band size : 71 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TRIM25 antibody (ab88669) at 1 µg/ml

    Lane 1 : TRIM25 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 71 kDa
    Observed band size : 71 kDa
  • Immunofluorescence of purified ab88669 staining TRIM25 on HeLa cell (antibody concentration 10 ug/ml)

参考文献

ab88669 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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