The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
Use at an assay dependent dilution.
機能Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
パスウェイProtein modification; protein ubiquitination.
ドメインThe RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity. The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
細胞内局在Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.