製品の概要

  • 製品名Anti-TRIM25 antibody
    TRIM25 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRIM25
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide, conjugated to KLH, containing a 19 amino acid sequence from the C terminal region of Human TRIM25

  • ポジティブ・コントロール
    • Human brain (cortex) tissue; HeLa cell lysate; HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use a concentration of 20 µg/ml.

ターゲット情報

  • 機能Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
  • 組織特異性Ubiquitous.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • ドメインThe RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
    The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
  • 翻訳後修飾Auto-ISGylated.
  • 細胞内局在Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin/ISG15 ligase TRIM25 antibody
    • EFP antibody
    • Estrogen responsive finger protein antibody
    • Estrogen-responsive finger protein antibody
    • RING finger protein 147 antibody
    • RNF 147 antibody
    • RNF147 antibody
    • TRI25 antibody
    • TRI25_HUMAN antibody
    • TRIM 25 antibody
    • Trim25 antibody
    • Tripartite motif containing 25 antibody
    • Tripartite motif containing protein 25 antibody
    • Tripartite motif protein TRIM25 antibody
    • Tripartite motif-containing protein 25 antibody
    • Tripartite motifcontaining25 antibody
    • Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody
    • Z147 antibody
    • Zinc finger protein 147 (estrogen responsive finger protein) antibody
    • Zinc finger protein 147 antibody
    • Zinc finger protein-147 antibody
    • ZNF 147 antibody
    • ZNF147 antibody
    see all

Anti-TRIM25 antibody 画像

  • All lanes : Anti-TRIM25 antibody (ab115737) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with blocking peptide


    Predicted band size : 71 kDa
  • ab115737 at 10ug/ml staining TRIM25 in Human brain (cortex) tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115737 at 20ug/ml staining TRIM25 by immunocytochemistry.

Anti-TRIM25 antibody (ab115737) 使用論文

ab115737 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab115737.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"