製品の概要

  • 製品名Anti-TRIM16 antibody
    TRIM16 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRIM16
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Horse, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human TRIM16, NP_006461.3.

  • ポジティブ・コントロール
    • Whole cell lysate from Hela, 293T and NIH 3T3 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能May play a role in the regulation of keratinocyte differentiation.
  • 組織特異性Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
  • 配列類似性Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EBBP antibody
    • Estrogen responsive B box protein antibody
    • Estrogen-responsive B box protein antibody
    • TRI16_HUMAN antibody
    • TRIM 16 antibody
    • TRIM16 antibody
    • Tripartite motif containing 16 antibody
    • Tripartite motif containing protein 16 antibody
    • Tripartite motif-containing protein 16 antibody
    see all

Anti-TRIM16 antibody 画像

  • All lanes : Anti-TRIM16 antibody (ab72129) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of TRIM16.
    Lane 1: ab72129 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab72129 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 10 seconds.

Anti-TRIM16 antibody (ab72129) 使用論文

ab72129 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"