Anti-TRIM16 抗体 (ab72129)
Key features and details
- Rabbit polyclonal to TRIM16
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRIM16 antibody
TRIM16 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRIM16 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide corresponding to a region between residues 1 and 50 of human TRIM16, NP_006461.3.
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ポジティブ・コントロール
- Whole cell lysate from Hela, 293T and NIH 3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72129の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
May play a role in the regulation of keratinocyte differentiation. -
組織特異性
Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 10626 Human
- Entrez Gene: 94092 Mouse
- Entrez Gene: 100173399 Orangutan
- Omim: 609505 Human
- SwissProt: O95361 Human
- SwissProt: Q99PP9 Mouse
- SwissProt: Q5R760 Orangutan
- Unigene: 123534 Human
see all -
別名
- EBBP antibody
- Estrogen responsive B box protein antibody
- Estrogen-responsive B box protein antibody
see all
画像
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All lanes : Anti-TRIM16 antibody (ab72129) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Immunoprecipitation/ Western Blot of TRIM16.
Lane 1: ab72129 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72129 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab72129 は 2 報の論文で使用されています。
- Wang Y et al. CircPTK2 inhibits cell cisplatin (CDDP) resistance by targeting miR-942/TRIM16 axis in non-small cell lung cancer (NSCLC). Bioengineered 13:3651-3664 (2022). PubMed: 35230201
- Wang N & Zhang T Downregulation of MicroRNA-135 Promotes Sensitivity of Non-Small Cell Lung Cancer to Gefitinib by Targeting TRIM16. Oncol Res 26:1005-1014 (2018). PubMed: 29295721