製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRIM14
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 204-253 (SFEPVKSFFK GLVEAVESTL QTPLDIRLSP TNHAYSALKV SSPRLVSNRP) of Human TRIM14, isoform beta (NP_150090).

  • ポジティブ・コントロール
    • Human fetal liver lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Inhibits the transcriptional activity of SPI1 in a dose-dependent manner.
  • 組織特異性
    Highest expression in liver; undetectable in skeletal muscle.
  • 配列類似性
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
  • ドメイン
    The B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5830400N10Rik antibody
    • KIAA0129 antibody
    • pub antibody
    • TRI14_HUMAN antibody
    • TRIM14 antibody
    • tripartite motif protein 14 antibody
    • tripartite motif protein TRIM14 antibody
    • tripartite motif-containing 14 antibody
    • Tripartite motif-containing protein 14 antibody
    see all

画像

  • Anti-TRIM14 antibody (ab85374) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa

プロトコール

参考文献

ab85374 has not yet been referenced specifically in any publications.

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