製品の概要

  • 製品名Triiodothyronine (T3) ELISA Kit
    Triiodothyronine (T3) キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 40 < 10.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 < 9.1%
  • テスト
    1 x 96 test
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    0.05 ng/ml
  • 添加回収試験

    97.5 %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.


     


    A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab108685 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Competitive ELISA Use at an assay dependent dilution.

    Triiodothyronine (T3) ELISA Kit 画像

    • Representative Standard Curve using ab108685

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    Triiodothyronine (T3) ELISA Kit (ab108685) 使用論文

    ab108685 has not yet been referenced specifically in any publications.

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