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Triglyceride Quantification Assay Kit (ab65336) provides a sensitive, easy assay to measure triglyceride (TG) concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a product which reacts with the probe to generate color (spectrophotometry at ?= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
If your sample contains reducing substances such as glucose, fructose or lactose, they are likely to interfere with the assay. In this case, we recommend to use PicoProbe Triglyceride Quantification Assay Kit (Fluorometric) (ab178780).
Visit our FAQs page for tips and troubleshooting.
This kit also detects monoglycerides and diglycerides.
If you need to purchase additional lipase for this product, please see ab89001.
If you need to purchase additional enzyme mix for this product, please see ab155160.
If you need to purchase additional TG standard, please see ab103967
If you need to purchase additional TG probe please see ab171898.
|Triglyceride Assay Buffer||1 x 25ml|
|Triglyceride Enzyme Mix (lyophilized)||Green||1 vial|
|Triglyceride Probe (in DMSO, anhydrous)||Red||1 x 200µl|
|Triglyceride Standard (1 mM)||Yellow||1 x 300µl|
Our Abpromise guarantee covers the use of ab65336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Fluorometric triglyceride standard curve: mean of duplicates (+/- SD) with background reads subtracted
Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.
HepG2 cells were treated with 25 uM Chloroquine for 72h.
Colorimetric triglyceride standard curve using ab65336.