製品の概要

  • 製品名
    Anti-Trichohyalin antibody [AE15]
    Trichohyalin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AE15] to Trichohyalin
  • 特異性
    This antibody recognises a 220K trichohyalin granular protein, a marker for hair follicular differentiation.
  • アプリケーション
    適用あり: WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Urea-mercaptoethanol extract of human hair follicles.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    AE15
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 220 kDa.
IHC-Fr Use at an assay dependent dilution. PubMed: 19786578
IHC-P Use at an assay dependent dilution. PubMed: 19038788

ターゲット情報

  • 機能
    Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.
  • 組織特異性
    Found in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium.
  • 配列類似性
    Belongs to the S100-fused protein family.
    Contains 2 EF-hand domains.
  • 発生段階
    Expressed during late differentiation of the epidermis.
  • ドメイン
    Consists of nine domains. Domain 1 contains two EF-hand calcium-binding domains. Domains 2-4, 6, and 8 are almost entirely alpha-helical, configured as a series of peptide repeats of varying regularity, and are thought to form a single-stranded alpha-helical rod stabilized by ionic interactions. Domain 6 is the most regular and may bind KIF directly by ionic interactions. Domains 5 and 7 are less well organized and may induce folds in the molecule. Domain 9 contains the C-terminus, conserved among different species.
  • 翻訳後修飾
    Substrate of transglutaminase. Some 200 arginines are probably converted to citrullines by peptidylarginine deimidase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 220K trichohyalin granular protein antibody
    • Hair follicle protein AHF antibody
    • MGC157889 antibody
    • MGC157890 antibody
    • RP11-107M16.3 antibody
    • TCHH antibody
    • THH antibody
    • THL antibody
    • TRHY_HUMAN antibody
    • Trichohyalin antibody
    • Trichohyalin granular protein antibody
    see all

画像

  • Anti-Trichohyalin antibody [AE15] (ab58755) at 1/100 dilution + Lung (Human) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 220 kDa
    Observed band size : 220 kDa
    Additional bands at : 165 kDa. We are unsure as to the identity of these extra bands.
  • ab58755 staining Trichohyalin in mouse skin tissue sections by Immunohistochemistry (IHC-Fr - paraformaldehyde-fixed, frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with a mouse blocking reagent for 1 hour at 25°C. The sample was incubated with primary antibody (1/100 in Diluent) at 4°C for 12 hours. An Alexa Fluor®546-conjugated Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody.

    See Abreview

  • ab58755 staining Trichohyalin in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rabbit . Read more (PubMed: 28190929) »
  • Imsland F  et al. Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horses. Nat Genet 48:152-8 (2016). Read more (PubMed: 26691985) »

See all 8 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
M.O.M. Mouse Ig Blocking Reagent (Vector Laboratories) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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