製品の概要

  • 製品名Anti-TRIB3 antibody [EPR3151Y]
    TRIB3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3151Y] to TRIB3
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues on the C terminus of human TRIB3

  • ポジティブ・コントロール
    • 293T cell lysates, untreated and treated with Ionomycin and TPA.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP 1/10.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
    • 組織特異性Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
    • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
      Contains 1 protein kinase domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C20orf97 antibody
      • Neuronal cell death inducible putative kinase antibody
      • Neuronal cell death-inducible putative kinase antibody
      • NIPK antibody
      • p65 interacting inhibitor of NF-kappaB antibody
      • p65-interacting inhibitor of NF-kappa-B antibody
      • SINK antibody
      • SKIP 3 antibody
      • SKIP3 antibody
      • TRB 3 antibody
      • TRB-3 antibody
      • TRB3 antibody
      • TRIB 3 antibody
      • Trib3 antibody
      • TRIB3_HUMAN antibody
      • Tribbles homolog 3 antibody
      • Tribbles pseudokinase 3 antibody
      • Tribbles3 antibody
      see all

    Anti-TRIB3 antibody [EPR3151Y] 画像

    • All lanes : Anti-TRIB3 antibody [EPR3151Y] (ab75846) at 1/10000 dilution

      Lane 1 : 293T cell lysates, treated with Ionomycin and TPA
      Lane 2 : 293T cell lysates, untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa

    Anti-TRIB3 antibody [EPR3151Y] (ab75846) 使用論文

    This product has been referenced in:
    • Boisson B  et al. Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency. Nat Immunol 13:1178-86 (2012). Human . Read more (PubMed: 23104095) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.  Both of these anti-TRIB3 antibodies has been tested for immunoprecipiation and are guaranteed for its use in this application. They have not been tested side by side and so we have no data on whether one may work better fo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"