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Our Abpromise guarantee covers the use of ab50516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. Staining of the TRB3 band is specifically inhibited with the immunizing peptide.|
|IP||Use at an assay dependent concentration.|
ab50516 staining TRIB3 in human brain tumor tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and permeabilized 0.5% TX-100 before blocking with 10% serum for 1 hour 30 minutes at 250C. The sample was incubated with primary antibody (1/200) in 1% serum in PBS for 2 hours at 250C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution.