Anti-TRIB3 抗体 - Aminoterminal end (ab50516)
Key features and details
- Rabbit polyclonal to TRIB3 - Aminoterminal end
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TRIB3 antibody - Aminoterminal end
TRIB3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRIB3 - Aminoterminal end -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow -
免疫原
Synthetic peptide:
GSLSRKKRLELDDNLDTER
, corresponding to N terminal amino acids 11-29 of Human TRIB3 conjugated to KLH -
ポジティブ・コントロール
- HEK-293 cells expressing human TRB3.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab50516 is affinity purified using the immunizing peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50516の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. Staining of the TRB3 band is specifically inhibited with the immunizing peptide.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. Staining of the TRB3 band is specifically inhibited with the immunizing peptide. |
ターゲット情報
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機能
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. -
組織特異性
Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 57761 Human
- Omim: 607898 Human
- SwissProt: Q96RU7 Human
- Unigene: 516826 Human
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別名
- C20orf97 antibody
- Neuronal cell death inducible putative kinase antibody
- Neuronal cell death-inducible putative kinase antibody
see all
画像
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All lanes : Anti-TRIB3 antibody - Aminoterminal end (ab50516) at 1/1000 dilution
Lane 1 : HEK293 cells transfected with human TRIB3
Lane 2 : HEK293 cells (untransfected)
Lane 3 : HEK293 cells transfected with human TRIB3
with TRIB3 immunizing peptide at 20 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 40 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab50516 は 5 報の論文で使用されています。
- Muñoz-Guardiola P et al. The anti-cancer drug ABTL0812 induces ER stress-mediated cytotoxic autophagy by increasing dihydroceramide levels in cancer cells. Autophagy 17:1349-1366 (2021). PubMed: 32397857
- Felip I et al. Therapeutic potential of the new TRIB3-mediated cell autophagy anticancer drug ABTL0812 in endometrial cancer. Gynecol Oncol 153:425-435 (2019). PubMed: 30853360
- Hu F et al. Knockdown of TRB3 improved the MPP+/MPTP-induced Parkinson's disease through the MAPK and AKT signaling pathways. Neurosci Lett 709:134352 (2019). PubMed: 31283965
- Guan H et al. Competition between members of the tribbles pseudokinase protein family shapes their interactions with mitogen activated protein kinase pathways. Sci Rep 6:32667 (2016). PubMed: 27600771
- Salazar M et al. Cannabinoid action induces autophagy-mediated cell death through stimulation of ER stress in human glioma cells. J Clin Invest 119:1359-72 (2009). IP ; Human . PubMed: 19425170