製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRIB3
  • アプリケーション
    適用あり: WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide from the N-terminal domain of human TRIB3

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 40 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
  • 組織特異性
    Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
  • 配列類似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C20orf97 antibody
    • Neuronal cell death inducible putative kinase antibody
    • Neuronal cell death-inducible putative kinase antibody
    • NIPK antibody
    • p65 interacting inhibitor of NF-kappaB antibody
    • p65-interacting inhibitor of NF-kappa-B antibody
    • SINK antibody
    • SKIP 3 antibody
    • SKIP3 antibody
    • TRB 3 antibody
    • TRB-3 antibody
    • TRB3 antibody
    • TRIB 3 antibody
    • Trib3 antibody
    • TRIB3_HUMAN antibody
    • Tribbles homolog 3 antibody
    • Tribbles pseudokinase 3 antibody
    • Tribbles3 antibody
    see all

画像

  • Anti-TRIB3 antibody (ab73547) at 1/500 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • All lanes : Anti-TRIB3 antibody (ab73547) at 1/1000 dilution

    Lane 1 : Lysate prepared from rat soleus tissue, homogenized in TPER
    Lane 2 : Lysate prepared from rat soleus tissue, homogenized in TPER
    Lane 3 : Lysate prepared from rat liver tissue, homogenized in TPER

    Lysates/proteins at 60 µg per lane.

    Secondary
    rabbit monoclonal conjugated to HRP at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

参考文献

This product has been referenced in:
  • Rzymski T  et al. Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4. Oncogene 27:4532-43 (2008). WB ; Human . Read more (PubMed: 18408768) »

See 1 Publication for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neural stem cell)
Permeabilization
Yes - 0.2% Triton-X
Specification
neural stem cell
Blocking step
Serum as blocking agent for 3 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
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投稿 Apr 19 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (soleus and liver)
Loading amount
60 µg
Specification
soleus and liver
Treatment
Homogenized in TPER (Pierce) Buffer
Gel Running Conditions
Reduced Denaturing (7% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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