製品の概要

  • 製品名Anti-TRF2 antibody
    TRF2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to TRF2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Baculovirus expressed His-tagged whole length TRF2 protein was used for immunizing goat.

  • ポジティブ・コントロール
    • Human cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use 2µg for 106 cells.
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 60 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
    • 組織特異性Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
    • 配列類似性Contains 1 HTH myb-type DNA-binding domain.
    • ドメインThe TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Telomeric DNA binding protein antibody
      • Telomeric DNA-binding protein antibody
      • Telomeric repeat binding factor 2 antibody
      • Telomeric repeat binding protein 2 antibody
      • Telomeric repeat-binding factor 2 antibody
      • TERF 2 antibody
      • Terf2 antibody
      • TERF2_HUMAN antibody
      • TRBF 2 antibody
      • TRBF2 antibody
      • TRF 2 antibody
      • TTAGGG repeat binding factor 2 antibody
      • TTAGGG repeat-binding factor 2 antibody
      see all

    Anti-TRF2 antibody 画像

    • Immunoprecipitation (IP)/ Western Blot (WB) Analysis of TRF2 in human cell lysates. Lane 1. IP with TRF2 antibody (mouse ant-TRF2). Lane 2. IP with control mouse IgG. Lane 3. IP with TRF2 antibody (goat anti-TRF2). Lane 4. IP with pre-immune goat Ig. Lanes 1-4. WB with TRF2 antibody. TRF2 is detected as an ~ 66 kD protein.

    Anti-TRF2 antibody (ab13589) 使用論文

    This product has been referenced in:
    • Wijeratne SS  et al. K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts. J Nutr Biochem 21:310-6 (2010). WB ; Human . Read more (PubMed: 19369050) »

    See 1 Publication for this product

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