製品の概要

  • 製品名Anti-TRF1 (phospho S219) antibody
    TRF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRF1 (phospho S219)
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthesized phosphopeptide derived from human TRF1 around the phosphorylation site of serine 219 (I-I-SP-Q-K).

  • ポジティブ・コントロール
    • 293 lysate treated with paclitaxel (1 µM, 24 hours)

製品の特性

  • 製品の状態Liquid
  • 保存方法Frozen Stock (-20C). Shelf life 12 months.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab79187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ELISA 1/40000.

ターゲット情報

  • 機能Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • 組織特異性Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • 配列類似性Contains 1 HTH myb-type DNA-binding domain.
  • ドメインThe acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • 翻訳後修飾Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • 細胞内局在Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hTRF1 AS antibody
    • NIMA interacting protein 2 antibody
    • NIMA-interacting protein 2 antibody
    • PIN 2 antibody
    • PIN2 antibody
    • t TRF1 antibody
    • Telomeric protein Pin2 antibody
    • Telomeric protein Pin2/TRF1 antibody
    • Telomeric repeat binding factor (NIMA interacting) 1 antibody
    • Telomeric repeat binding factor 1 antibody
    • Telomeric repeat binding protein 1 antibody
    • Telomeric repeat-binding factor 1 antibody
    • TERF 1 antibody
    • Terf1 antibody
    • TERF1_HUMAN antibody
    • TRBF 1 antibody
    • TRBF1 antibody
    • TRF 1 antibody
    • TRF antibody
    • TTAGGG repeat binding factor 1 antibody
    • TTAGGG repeat-binding factor 1 antibody
    see all

Anti-TRF1 (phospho S219) antibody 画像

  • Lanes 1 - 2 : Anti-TRF1 (phospho S219) antibody (ab79187) at 1/500 dilution
    Lane 3 : A different TRF1 antibody (ab63374)

    Lane 1 : 293 cells treated with paclitaxel (1 µM, 24 hours)
    Lane 2 : 293 cells treated with paclitaxel (1 µM, 24 hours) with immunizing phosphopeptide at 10 µg
    Lane 3 : 293 cells treated with paclitaxel (1 µM, 24 hours)

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa

Anti-TRF1 (phospho S219) antibody (ab79187) 使用論文

ab79187 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"