製品の概要

  • 製品名Anti-TRF1 antibody [TRF-78]
    TRF1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TRF-78] to TRF1
  • 特異性We would not recommend to use this antibody with mouse samples as the homology of human and murine TRF1 is low (67%). Therefore, the cross-reactivity with the mouse protein is unlikely/inconsistent and cannot be guaranteed
  • アプリケーション適用あり: ELISA, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human TRF1 protein produce in baculovirus

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 50 kDa.
IHC-P 1/100. PubMed: 18641004
ICC/IF 1/100.

ターゲット情報

  • 機能Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • 組織特異性Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • 配列類似性Contains 1 HTH myb-type DNA-binding domain.
  • ドメインThe acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • 翻訳後修飾Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • 細胞内局在Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hTRF1 AS antibody
    • NIMA interacting protein 2 antibody
    • NIMA-interacting protein 2 antibody
    • PIN 2 antibody
    • PIN2 antibody
    • t TRF1 antibody
    • Telomeric protein Pin2 antibody
    • Telomeric protein Pin2/TRF1 antibody
    • Telomeric repeat binding factor (NIMA interacting) 1 antibody
    • Telomeric repeat binding factor 1 antibody
    • Telomeric repeat binding protein 1 antibody
    • Telomeric repeat-binding factor 1 antibody
    • TERF 1 antibody
    • Terf1 antibody
    • TERF1_HUMAN antibody
    • TRBF 1 antibody
    • TRBF1 antibody
    • TRF 1 antibody
    • TRF antibody
    • TTAGGG repeat binding factor 1 antibody
    • TTAGGG repeat-binding factor 1 antibody
    see all

Anti-TRF1 antibody [TRF-78] 画像

  • Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody.  A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation.  The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports.

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain. 

  • ab10579 at 1/500 staining human HeLa cells by ICC/IF. The cells were parafomaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 555 conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

Anti-TRF1 antibody [TRF-78] (ab10579) 使用論文

This product has been referenced in:
  • Popuri V  et al. Human RECQL1 participates in telomere maintenance. Nucleic Acids Res 42:5671-88 (2014). ICC/IF ; Human . Read more (PubMed: 24623817) »
  • Hopkins J  et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). ICC/IF ; Mouse . Read more (PubMed: 24992337) »

See all 17 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Brain)
Permeabilization Yes - Triton Tx 0,3%
Specification Brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Dr. Milan Stojiljkovic

Verified customer

投稿 Mar 10 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (HEK293T-TRF1 HEK293T-TRF2)
Gel Running Conditions Reduced Denaturing
Loading amount 100 µg
Specification HEK293T-TRF1 HEK293T-TRF2
Blocking step (agent) for 1 hour(s) and 10 minute(s) · Concentration: 2% · Temperature: 37°C
Username

giovanna roncador

Verified customer

投稿 Jul 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Mouse Cell (oocyte)
Specification oocyte
Permeabilization Yes - 0.01% triton-X100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 28 2014

Merci de nous avoir contactés.

En compensation des 4 unités de ab10579 qui n'ont pas fonctionné, j'ai mis en place l'envoi d'une unité de ab32084 et d'une unité de ab1838, les numéros de commandes sont...

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Thank you for your reply.

I hope the new vial will provide good results!

I am sorry that I have not seen the crossreactivity note on the datasheet of the secondary antibody.I might would nevertheless performa control slide using you...

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Thank you very much for having provided all these additional information.

I have seen the results and I agree that the staining is not as the previous staining. As for the reasons explained before, I just would like to ask you to confirm the ...

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Thank you for contacting us.

While to our knowledgethis TRF1 antibody has not been tested for cross-reactivity with TRF2, it is not likely to detect that protein. The homology between TRF1 and TRF2 is only around 17%. We have not had any cust...

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Thank you for contacting us.

At this time we have not determined the light chain type of this antibody, we apologize for any inconvenience.

I hope this information is helpful to you. Please do not hesitate to contact us if you...

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Thank you for contacting us.

For the antibodies with the clone numbers you mentioned we have the follwoing immunogen information:

BED5 57-6 - ab47717 (Human full length recombinant protein expressed in bacteria)

BED5 - ab...

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Thank you for your email.

Your credit note ID is xxx for ab10579.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, pl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"