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Highly purified Treponema pallidum.
Titer: >1:3,000 (indirect FA). Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated molar HRP:IgG substitution is 2-3. >95% pure, before conjugation.
Our Abpromise guarantee covers the use of ab20095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent dilution.|
ab20095 has not yet been referenced specifically in any publications.