製品の概要

  • 製品名
    Anti-TREM2 antibody [RM0139-5J46]
    TREM2 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RM0139-5J46] to TREM2
  • 特異性
    This antibody detects mouse TREM2, but not TREM1 in Western blotting.
  • アプリケーション
    適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Mouse recombinant protein of the extracellular EC-domain of TREM2

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    The IgG fraction of culture supernatant was purified by Protein A affinity chromatography.
  • ポリ/モノ
    モノクローナル
  • クローン名
    RM0139-5J46
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/250.
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.

ターゲット情報

  • 機能
    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
  • 組織特異性
    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
  • 関連疾患
    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • 配列類似性
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • TREM 2 antibody
    • TREM-2 antibody
    • TREM2 antibody
    • TREM2_HUMAN antibody
    • TREM2a antibody
    • TREM2b antibody
    • TREM2c antibody
    • Trggering receptor expressed on myeloid cells 2 antibody
    • Trggering receptor expressed on myeloid cells 2a antibody
    • Triggering receptor expressed on monocytes 2 antibody
    • Triggering receptor expressed on myeloid cells 2 antibody
    see all

Anti-TREM2 antibody [RM0139-5J46] 画像

  • Immunofluorescence analysis of 18 day rat embroyonic hypothalmic neurones labelling TREM2 with ab86491. Embryonic hypothalmic neuronal cells were cultured in a neurobasal media with B27 culture suppliment for three weeks and fixed with 2%PFA. A rat monoclonal Alexa fluoro® 568 (red) secondary was used at a concentration of 1/1000. Synaptophysin was counterstained with an Alexa fluoro® 488 (green) and nuclear DNA was stained with DAPI (blue).

     

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  • IHC-Fr image of human blood vessels stained with Ab86491 (1:250). The fixed brain sections were permeabilized using 0.1% PBS-Triton X for 1h. 10% donkey serum was used for 1 hour at 24°C to block protein-protein interactions.  The sections were then incubated with Ab8649 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-rat used at 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Anti-TREM2 antibody [RM0139-5J46] (ab86491) 使用論文

This product has been referenced in:
  • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). WB, IHC ; Mouse . Read more (PubMed: 27662313) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal neuron)
Permeabilization
Yes - 0.1 M PBS with 1% Triton X
Specification
Hippocampal neuron
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 21 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Serum (Human Serum)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
Human Serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 20 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Tissue sections (Blood vessels)
Specification
Blood vessels
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Formaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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