製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to TREM2
  • アプリケーション
    適用あり: ELISA, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    C-HGQKPGTHPPSELD

    by a Cysteine residue linker, corresponding to C terminal amino acids 199-212 of Human TREM2

  • ポジティブ・コントロール
    • Human spleen macrophages

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/32000.
IHC-P Use a concentration of 2.5 µg/ml.
IHC-Fr 1/50. (see Abreview, worked well in human brain but not rabbit brain sections)

ターゲット情報

  • 機能
    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
  • 組織特異性
    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
  • 関連疾患
    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • 配列類似性
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • TREM 2 antibody
    • TREM-2 antibody
    • TREM2 antibody
    • TREM2_HUMAN antibody
    • TREM2a antibody
    • TREM2b antibody
    • TREM2c antibody
    • Trggering receptor expressed on myeloid cells 2 antibody
    • Trggering receptor expressed on myeloid cells 2a antibody
    • Triggering receptor expressed on monocytes 2 antibody
    • Triggering receptor expressed on myeloid cells 2 antibody
    see all

画像

  • ab85851 at 2.5 µg/ml staining TREM2 in Human Spleen macrophages in formalin fixed, paraffin embedded tissue.

プロトコール

参考文献

This product has been referenced in:
  • Satoh J  et al. A survey of TREM2 antibodies reveals neuronal but not microglial staining in formalin-fixed paraffin-embedded postmortem Alzheimer's brain tissues. Alzheimers Res Ther 5:30 (2013). Read more (PubMed: 23830013) »

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Mouse brain tissues)
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Specification
Mouse brain tissues
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 01 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
Yes - triton x(20ul/10mlPBS)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Praveen Ballabh

Verified customer

投稿 Sep 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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