リコンビナント
RabMAb

Anti-Trefoil Factor 3 抗体 [EPR3973] (ab109104)

製品の概要

  • 製品名
    Anti-Trefoil Factor 3 antibody [EPR3973]
    Trefoil Factor 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3973] to Trefoil Factor 3
  • アプリケーション
    適用あり: WB, IHC-P, ICC, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Trefoil Factor 3.

  • ポジティブ・コントロール
    • Human uterus cancer and colon cancer lysates; Human colon tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
    • 組織特異性
      Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
    • 配列類似性
      Contains 1 P-type (trefoil) domain.
    • 細胞内局在
      Secreted > extracellular space > extracellular matrix > apical lamina.
    • Information by UniProt
    • 参照データベース
    • 別名
      • hITF antibody
      • hP1.B antibody
      • Intestinal trefoil factor antibody
      • ITF antibody
      • mITF antibody
      • OTTMUSP00000021729 antibody
      • P1B antibody
      • Polypeptide P1.B antibody
      • TFF3 antibody
      • TFF3_HUMAN antibody
      • TFI antibody
      • TREFOIL antibody
      • Trefoil factor (intestinal) antibody
      • Trefoil factor 3 antibody
      see all

    Anti-Trefoil Factor 3 antibody [EPR3973] 画像

    • All lanes : Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104) at 1/1000 dilution

      Lane 1 : Human uterus cancer lysate
      Lane 2 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 9 kDa
    • Immunohistochemical analysis of Trefoil Factor 3 in paraffin-embedded Human colon tissue using ab109104 at 1/500 dilution.
    • Flow Cytometry analysis of HT-29 (human colorectal adenocarcinoma) cells labeling Trefoil Factor 3 with unpurified ab109104 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104) 使用論文

    This product has been referenced in:
    • Derakhshan MH  et al. In healthy volunteers, immunohistochemistry supports squamous to columnar metaplasia as mechanism of expansion of cardia, aggravated by central obesity. Gut 64:1705-14 (2015). Read more (PubMed: 25753030) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Serum (Mouse Serum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Mouse Serum
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 28 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録