製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml.

ターゲット情報

  • 機能Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
  • 組織特異性Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
  • 配列類似性Contains 1 P-type (trefoil) domain.
  • 細胞内局在Secreted > extracellular space > extracellular matrix > apical lamina.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hITF antibody
    • hP1.B antibody
    • Intestinal trefoil factor antibody
    • ITF antibody
    • mITF antibody
    • OTTMUSP00000021729 antibody
    • P1B antibody
    • Polypeptide P1.B antibody
    • TFF3 antibody
    • TFF3_HUMAN antibody
    • TFI antibody
    • TREFOIL antibody
    • Trefoil factor (intestinal) antibody
    • Trefoil factor 3 antibody
    see all

Anti-Trefoil Factor 3 antibody 画像

  • Trefoil Factor 3 antibody (ab57752) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
  • Western blot against tagged recombinant protein immunogen using ab57752 Trefoil Factor 3 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa

Anti-Trefoil Factor 3 antibody (ab57752) 使用論文

This product has been referenced in:
  • Ding A  et al. Impact of NPM, TFF3 and TACC1 on the prognosis of patients with primary gastric cancer. PLoS One 8:e82136 (2013). IHC-P ; Human . Read more (PubMed: 24358147) »
  • Iwasaki M  et al. Longitudinal cell formation in the entire human small intestine is correlated with the localization of Hath1 and Klf4. J Gastroenterol 46:191-202 (2011). IHC-P ; Human . Read more (PubMed: 21125297) »

See all 3 Publications for this product

Product Wall

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This kit will work with mouse IgM antiobodies as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoc...

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Thank you for contacting us.

ab127055 kit can be used with any mouse antibody - this mean the kit would be suitable.

Please note ab49536 is tested and guaranteed to be with mouse species however ab57752 is only tested with human sampl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT-29)
Loading amount 10 µg
Specification HT-29
Treatment IP
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Ms. qianxue wan

Verified customer

投稿 Oct 10 2012

Thank you for your enquiry.
You are correct that both ab108599 and ab57752 are both monoclonal antibodies designed to detect tff3. In additiona they have both been found to detect human tff3 via WB and IHC-P. Other than these characteristics, thar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"