Anti-Trefoil Factor 3 抗体 (ab101099)

製品の概要

  • 製品名
    Anti-Trefoil Factor 3 antibody
    Trefoil Factor 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Trefoil Factor 3
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Sheep, Cow, Pig, Macaque monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human Trefoil Factor 3 .

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human normal colon FFPE section.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
  • 組織特異性
    Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
  • 配列類似性
    Contains 1 P-type (trefoil) domain.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix > apical lamina.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hITF antibody
    • hP1.B antibody
    • Intestinal trefoil factor antibody
    • ITF antibody
    • mITF antibody
    • OTTMUSP00000021729 antibody
    • P1B antibody
    • Polypeptide P1.B antibody
    • TFF3 antibody
    • TFF3_HUMAN antibody
    • TFI antibody
    • TREFOIL antibody
    • Trefoil factor (intestinal) antibody
    • Trefoil factor 3 antibody
    see all

Anti-Trefoil Factor 3 antibody 画像

  • IHC image of Trefoil Factor 3 staining in human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101099, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ICC/IF image of ab101099 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101099 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Trefoil Factor 3 antibody (ab101099) 使用論文

ab101099 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Cat Tissue lysate - other (feces)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% polyacrylamide gel)
Loading amount
36 µg
Specification
feces
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jul 25 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Sheep Lung Tissue)
Antigen retrieval step
Other
Permeabilization
Yes - Used regular TBS-T buffer
Specification
Sheep Lung Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 26°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録