Limited availability. The term 'limited availability' is employed where there is only a small amount of stock available for that antibody. Despite the limited stock, we have released the product for sale because we believe it may be of use in your research.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
All lanes : Anti-TRAX antibody (ab24334) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa Observed band size: 33 kDa Additional bands at: 51 kDa, 63 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab24334 detects a strong band at ~ 33 kDa. This corresponds to the predicted molecular weight for TRAX. Some weaker bands are also detected at ~ 51 and 63 kDa. We are unsure as to the nature of these bands but predict they represent non-specific binding.