製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRAPPC6A
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 42-72 of Human TRAPPC6A.

  • ポジティブ・コントロール
    • Jurkat cell line lysates; Human lung carcinoma tissue. IF/ICC: A549 cell line.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab103844 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    May play a role in vesicular transport during the biogenesis of melanosomes.
  • 配列類似性
    Belongs to the TRAPP small subunits family. BET3 subfamily.
  • 細胞内局在
    Golgi apparatus > cis-Golgi network. Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1810073E21Rik antibody
    • 4930519D19Rik antibody
    • AI480686 antibody
    • HSPC289 antibody
    • MGC117907 antibody
    • mhyp antibody
    • OTTMUSP00000025165 antibody
    • OTTMUSP00000025166 antibody
    • rCG_54352 antibody
    • RGD1309160 antibody
    • TPC6A_HUMAN antibody
    • Trafficking protein particle complex 6A antibody
    • Trafficking protein particle complex subunit 6A antibody
    • TRAPP complex subunit 6A antibody
    • TRAPPC6A antibody
    • TRS33 antibody
    see all

画像

  • ab103844 stained A549 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103844 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-TRAPPC6A antibody (ab103844) at 1/100 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size : 18 kDa
  • ab103844 at 1/50 dilution, staining TRAPPC6A in Human lung carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

参考文献

ab103844 has not yet been referenced specifically in any publications.

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