Rabbit polyclonal to TRAPPC4 - Aminoterminal end
Synthetic peptide corresponding to Human TRAPPC4 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
NULL cell lysate
Human lung carcinoma tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Protein A purified
This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
1/10 - 1/50.
TRAPPC4 is part of the multisubunit TRAPP (transport protein particle) complex and interacts with SDC2. It may play a role in vesicular transport from endoplasmic reticulum to Golgi. TRAPP proteins are involved in tethering during vesicle transport.
Endoplasmic reticulum and Golgi Apparatus
CGI 104 antibody
Hematopoietic stem/progenitor cell protein 172 antibody
Western blot - TRAPPC4 antibody (ab70906)
Anti-TRAPPC4 antibody - Aminoterminal end (ab70906) at 1/60 dilution + NULL cell lysate at 35 µg
Predicted band size: 24 kDa Observed band size: 24 kDa Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRAPPC4 antibody (ab70906)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue labelled with ab70906 at 1/10 dilution followed by peroxidase conjugated secondary antibody use and DAB staining.
has not yet been referenced specifically in any publications.
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