製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRAP80
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 331-361 of Human TRAP80 (NP_004259.3).

  • ポジティブ・コントロール
    • MDA-MB231 cell line lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 73 kDa.

ターゲット情報

  • 機能
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 組織特異性
    Ubiquitous.
  • 関連疾患
    Defects in MED17 are the cause of microcephaly postnatal progressive with seizures and brain atrophy (MCPHSBA) [MIM:613668]. It is a disorder characterized by postnatal progressive microcephaly and severe developmental retardation associated with cerebral and cerebellar atrophy. Infants manifest swallowing difficulties leading to failure to thrive, jitteriness, poor visual fixation, truncal arching, seizures. There is no acquisition of developmental milestones and patients suffer from marked spasticity and profound retardation. Progressive microcephaly becomes evident few months after birth.
  • 配列類似性
    Belongs to the Mediator complex subunit 17 family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 77-KD subunit antibody
    • Activator recruited cofactor 77 kDa component antibody
    • Activator-recruited cofactor 77 kDa component antibody
    • ARC77 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 6 antibody
    • cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa antibody
    • CRSP complex subunit 6 antibody
    • CRSP6 antibody
    • CRSP77 antibody
    • DRIP80 antibody
    • med17 antibody
    • MED17_HUMAN antibody
    • Mediator complex subunit 17 antibody
    • Mediator of RNA polymerase II transcription subunit 17 antibody
    • Mediator of RNA polymerase II transcription subunit 17, S cerevisiae, homolog of antibody
    • Thyroid hormone receptor-associated protein complex 80 kDa component antibody
    • Thyroid hormone receptor-associated protein, 80-KD subunit antibody
    • Transcriptional coactivator CRSP77 antibody
    • Trap80 antibody
    • VITAMIN D RECEPTOR-INTERACTING PROTEIN, 80-KD antibody
    • Vitamin D3 receptor-interacting protein complex 80 kDa component antibody
    see all

画像

  • Anti-TRAP80 antibody (ab124176) at 1/100 dilution + MDA MB231 cell line lysate at 35 µg

    Predicted band size : 73 kDa

プロトコール

参考文献

ab124176 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
10 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

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投稿 Nov 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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