製品の概要

  • 製品名Anti-TRAP220/MED1 antibody
    TRAP220/MED1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRAP220/MED1
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from internal region of human TRAP220/MED1

  • ポジティブ・コントロール
    • Human breast carcinoma tissue. Extracts from Jurkat cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).

ターゲット情報

  • 機能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the Mediator complex subunit 1 family.
  • 翻訳後修飾Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator-recruited cofactor 205 kDa component antibody
    • ARC205 antibody
    • CRSP1 antibody
    • CRSP200 antibody
    • DRIP205 antibody
    • DRIP230 antibody
    • MED1 antibody
    • MED1_HUMAN antibody
    • Mediator complex subunit 1 antibody
    • Mediator of RNA polymerase II transcription subunit 1 antibody
    • p53 regulatory protein RB18A antibody
    • PBP antibody
    • Peroxisome proliferator-activated receptor-binding protein antibody
    • PPAR binding protein antibody
    • PPAR-binding protein antibody
    • PPARBP antibody
    • PPARGBP antibody
    • RB18A antibody
    • Thyroid hormone receptor-associated protein complex 220 kDa component antibody
    • Thyroid receptor-interacting protein 2 antibody
    • TR-interacting protein 2 antibody
    • Trap220 antibody
    • TRIP-2 antibody
    • TRIP2 antibody
    • Vitamin D receptor-interacting protein complex component DRIP205 antibody
    see all

Anti-TRAP220/MED1 antibody 画像

  • All lanes : Anti-TRAP220/MED1 antibody (ab64965) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells, without immunising peptide
    Lane 2 : extracts from Jurkat cells, with immunising peptide


    Predicted band size : 180 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using 1/50 ab64965, after antigen retrieval by boiling in a pressure cooker for 2-3 min. Polymer system was used for signal enhancing and DAB staining was performed for visualising the signal.

Anti-TRAP220/MED1 antibody (ab64965) 使用論文

This product has been referenced in:
  • Huszar JM  et al. Med1 regulates meiotic progression during spermatogenesis in mice. Reproduction 149:597-604 (2015). IHC-P . Read more (PubMed: 25778538) »
  • Villar J  et al. Androgen-induced activation of gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) transcription: essential role of a nonclassical androgen response element half-site. Mol Cell Biol 32:1566-80 (2012). Read more (PubMed: 22331472) »

See all 5 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (U2OS cells)
Total protein in input 500 µg
Immuno-precipitation step Protein A/G
Specification U2OS cells
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投稿 Jul 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
Loading amount 20 µg
Specification MEF cells
Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Mar 28 2017

Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions Reduced Denaturing (10% Gel)
Loading amount 50 µg
Specification U2OS cells
Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Verified customer

投稿 Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"