製品の概要

  • 製品名Anti-TRAP100 antibody
    TRAP100 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to TRAP100
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Fusion protein:

    EFLLKLTPLLDKADQRCNCDCTNFLLQECNKQGLLSEVNFASLVGKRTAD RDPQLKSSENANIQPNPGLILRAEPTVTNILKTMDADHSKSPEGLLGVLG

    , corresponding to amino acids 308/407 of Mouse TRAP100.

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーConstituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab21933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 110 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 組織特異性Ubiquitous. Abundant in skeletal muscle, heart and placenta.
  • 配列類似性Belongs to the Mediator complex subunit 24 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CRSP complex subunit 4 antibody
    • Activator recruited cofactor 100 kDa component antibody
    • Activator-recruited cofactor 100 kDa component antibody
    • ARC100 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 4 antibody
    • CRSP complex subunit 4 antibody
    • CRSP100 antibody
    • CRSP4 antibody
    • DRIP100 antibody
    • hTRAP100 antibody
    • KIAA0130 antibody
    • MED 24 antibody
    • MED24 antibody
    • MED24_HUMAN antibody
    • Mediator complex subunit 24 antibody
    • Mediator of RNA polymerase II transcription subunit 24 antibody
    • Mediator of RNA polymerase II transcription subunit 24 homolog antibody
    • MGC8748 antibody
    • OTTHUMP00000164458 antibody
    • OTTHUMP00000164460 antibody
    • THRAP4 antibody
    • Thyroid hormone receptor associated protein 4 antibody
    • Thyroid hormone receptor associated protein complex 100 kDa component antibody
    • Thyroid hormone receptor-associated protein 4 antibody
    • Thyroid hormone receptor-associated protein complex 100 kDa component antibody
    • Trap100 antibody
    • Trapp100 antibody
    • Vitamin D3 receptor interacting protein complex component DRIP100 antibody
    • Vitamin D3 receptor-interacting protein complex 100 kDa component antibody
    see all

Anti-TRAP100 antibody 画像

  • All lanes : Anti-TRAP100 antibody (ab21933) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 110 kDa

Anti-TRAP100 antibody (ab21933) 使用論文

ab21933 has not yet been referenced specifically in any publications.

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