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RabMAb

Anti-TRAP1 抗体 [EPR5381] (ab109323)

製品の概要

  • 製品名
    Anti-TRAP1 antibody [EPR5381]
    TRAP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5381] to TRAP1
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human TRAP1.

  • ポジティブ・コントロール
    • WB: K562, HL60, 293T and HeLa cell lysates IHC-P: Human kidney and liver tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/5000.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Chaperone that expresses an ATPase activity.
    • 組織特異性
      Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
    • 配列類似性
      Belongs to the heat shock protein 90 family.
    • 細胞内局在
      Mitochondrion.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Heat shock protein 75 kDa antibody
      • Heat shock protein 75 kDa, mitochondrial antibody
      • HSP 75 antibody
      • HSP75 antibody
      • HSP90L antibody
      • mitochondrial antibody
      • TNF receptor associated protein 1 antibody
      • TNFR-associated protein 1 antibody
      • TRAP-1 antibody
      • Trap1 antibody
      • TRAP1_HUMAN antibody
      • Tumor necrosis factor type 1 receptor-associated protein antibody
      see all

    Anti-TRAP1 antibody [EPR5381] 画像

    • All lanes : Anti-TRAP1 antibody [EPR5381] (ab109323) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 80 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human liver tissue by Immunohistochemistry.

    Anti-TRAP1 antibody [EPR5381] (ab109323) 使用論文

    This product has been referenced in:
    • Megger DA  et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). Read more (PubMed: 23462207) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109323.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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