リコンビナント
RabMAb

Anti-TRAP1 抗体 [EPR5381] (ab109323)

製品の概要

  • 製品名
    Anti-TRAP1 antibody [EPR5381]
    TRAP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5381] to TRAP1
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human TRAP1.

  • ポジティブ・コントロール
    • WB: K562, HL60, 293T and HeLa cell lysates IHC-P: Human kidney and liver tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/5000.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Chaperone that expresses an ATPase activity.
    • 組織特異性
      Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
    • 配列類似性
      Belongs to the heat shock protein 90 family.
    • 細胞内局在
      Mitochondrion.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Heat shock protein 75 kDa antibody
      • Heat shock protein 75 kDa, mitochondrial antibody
      • HSP 75 antibody
      • HSP75 antibody
      • HSP90L antibody
      • mitochondrial antibody
      • TNF receptor associated protein 1 antibody
      • TNFR-associated protein 1 antibody
      • TRAP-1 antibody
      • Trap1 antibody
      • TRAP1_HUMAN antibody
      • Tumor necrosis factor type 1 receptor-associated protein antibody
      see all

    画像

    • All lanes : Anti-TRAP1 antibody [EPR5381] (ab109323) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 80 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human liver tissue by Immunohistochemistry.

    参考文献

    This product has been referenced in:
    • Megger DA  et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). Read more (PubMed: 23462207) »

    See 1 Publication for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab109323.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録