製品の概要

  • 製品名
    TRAP-6
  • 製品の詳細
    PAR1 agonist
  • 別名
    • Thrombin Receptor Activator Peptide 6
  • 生理活性の詳細
    Peptide fragment (residues 42 - 47) of protease-activated receptor 1. PAR1 hexapeptide agonist. Stimulates transient mobilization of free intracellular calcium, platelet aggregation (EC50 = 0.8μM) and rapidly phosphorylates PDE3A.
  • 精製度
    > 96%

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • ab6672 staining IL6 in serum starved PC3 cells treated with TRAP-6 (ab120801), by ICC/IF. Increase in expression of IL6 correlates with increased concentration of TRAP-6, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120801 (TRAP-6) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6672 (1/500 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:
  • Picker SM  et al. Cell quality of apheresis-derived platelets treated with riboflavin-ultraviolet light after resuspension in platelet additive solution. Transfusion 52:510-6 (2012). Read more (PubMed: 21981030) »
  • Hunter RW  et al. Protein kinase C-mediated phosphorylation and activation of PDE3A regulate cAMP levels in human platelets. J Biol Chem 284:12339-48 (2009). Read more (PubMed: 19261611) »
  • Kaufmann R  et al. PAR 1-type thrombin receptors are involved in thrombin-induced calcium signaling in human meningioma cells. J Neurooncol 42:131-6 (1999). Read more (PubMed: 10421070) »
  • Vassallo RR  et al. Structure-function relationships in the activation of platelet thrombin receptors by receptor-derived peptides. J Biol Chem 267:6081-5 (1992). Read more (PubMed: 1313429) »

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