Tranylcypromine hydrochloride (2-PCPA) (ab120606)

製品の概要

  • 製品名Tranylcypromine hydrochloride (2-PCPA)
  • 製品の詳細
    Irreversible monoamine oxidase (MAO) inhibitor
  • 別名
    • 2-PCPA
  • 生理活性の詳細Irreversible monoamine oxidase (MAO) inhibitor. Increases serotonergic, noradrenergic activity and augments dopamine transmission. Additionally inhibits LSD1 (BHC110), inhibiting histone demethylation (IC50 = >50 μM). Anticonvulsant and antidepressant.
  • 精製度> 98%

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab120606 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Tranylcypromine hydrochloride (2-PCPA) 画像

    • MCF7 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of tranylcypromine hydrochloride (ab120606). Increased expression of Histone 3 K4 di-methyl (ab7766) in MCF7 cells correlates with an increase in tranylcypromine hydrochloride concentration, as described in literature.

      Nuclear extracts were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab7766 at 1 µg/ml and ab1791 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    Tranylcypromine hydrochloride (2-PCPA) (ab120606) 使用論文

    This product has been referenced in:
    • Hill MN  et al. Differential effects of the antidepressants tranylcypromine and fluoxetine on limbic cannabinoid receptor binding and endocannabinoid contents. J Neural Transm 115:1673-9 (2008). Read more (PubMed: 18974922) »
    • Schmidt DM & McCafferty DG trans-2-Phenylcyclopropylamine is a mechanism-based inactivator of the histone demethylase LSD1. Biochemistry 46:4408-16 (2007). Read more (PubMed: 17367163) »
    • Lee MG  et al. Histone H3 lysine 4 demethylation is a target of nonselective antidepressive medications. Chem Biol 13:563-7 (2006). Read more (PubMed: 16793513) »

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