Rabbit polyclonal to Transmembrane 4 L6 family member 1
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Transmembrane 4 L6 family member 1.
(Peptide available as
This antibody gave a positive signal in Human ovarian carcinoma tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
Highly expressed in lung, breast, colon and ovarian carcinomas. It is also present on some normal cells, endothelial cells in particular.
Belongs to the L6 tetraspanin family.
Information by UniProt
Membrane component chromosome 3 surface marker 1 antibody
Western blot - Transmembrane 4 L6 family member 1 antibody (ab79958)
Anti-Transmembrane 4 L6 family member 1 antibody (ab79958) at 1/1 dilution + Human ovary tumor tissue lysate (carcinoma) - insoluble fraction (female, 74 years) (
) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
24 kDa (
why is the actual band size different from the predicted?
Additional bands at :
100 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
Immunocytochemistry/ Immunofluorescence - Transmembrane 4 L6 family member 1 antibody (ab79958)
ICC/IF image of ab79958 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79958, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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