Human Transmembrane 4 L6 family member 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted as well as the banding pattern observed.
Immunocytochemistry/ Immunofluorescence - Transmembrane 4 L6 family member 1 antibody (ab79958)
ICC/IF image of ab79958 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79958, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.