製品の概要

  • 製品名Anti-Transglutaminase 3 antibody
    Transglutaminase 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Transglutaminase 3
  • 特異性This antibody is specific to Transglutaminase 3 from epidermal cell.
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 15 residue synthetic peptide derived from a domain specific for Transglutaminase 3 protein.

  • ポジティブ・コントロール
    • Human epidermal extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 21335491
WB 1/200 - 1/1000. Predicted molecular weight: 76 kDa.

ターゲット情報

  • 機能Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
  • 配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • 翻訳後修飾Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075).
  • Information by UniProt
  • 参照データベース
  • 別名
    • E polypeptide protein glutamine gamma glutamyltransferase antibody
    • MGC126249 antibody
    • MGC126250 antibody
    • Protein glutamine gamma glutamyltransferase E 27 kDa catalytic chain antibody
    • Protein glutamine gamma glutamyltransferase E 50 kDa noncatalytic chain antibody
    • Protein glutamine gamma glutamyltransferase E antibody
    • Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain antibody
    • TG(E) antibody
    • TGase E antibody
    • TGase-3 antibody
    • TGE antibody
    • TGM 3 antibody
    • TGM3 antibody
    • TGM3_HUMAN antibody
    • Transglutaminase E antibody
    • Transglutaminase-3 antibody
    • Transglutaminase3 antibody
    • TransglutaminaseE antibody
    see all

Anti-Transglutaminase 3 antibody 画像



  • Predicted band size : 76 kDa

Anti-Transglutaminase 3 antibody (ab27001) 使用論文

This product has been referenced in:
  • Zone JJ  et al. Dermatitis herpetiformis sera or goat anti-transglutaminase-3 transferred to human skin-grafted mice mimics dermatitis herpetiformis immunopathology. J Immunol 186:4474-80 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21335491) »

See 1 Publication for this product

Product Wall

The antibody was validated on different systems including keratinocytes. TG3 zymogen (77kDa) in skin is known to be cleaved (proteolysed) into 47 and 30 kDa. Indeed, this antibody recognizes both full length (77 kDa) and proteolysed form of 50kD.

Thank you for your enquiry. I have mailed the western image as an attachment in an e-mail that follows. Unfortunately the contrast on the blot is not as good as I would hope. The positive control used for the western blot was a Human epidermal extr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"