Anti-Transglutaminase 2 抗体 [EP2957] (ab109200)

製品の概要

  • 製品名Anti-Transglutaminase 2 antibody [EP2957]
    Transglutaminase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2957] to Transglutaminase 2
  • アプリケーション適用あり: WB, IP, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • ポジティブ・コントロール
    • U87-MG, A549 and HUVEC cell lysates; Human kidney tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • 配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

Anti-Transglutaminase 2 antibody [EP2957] 画像

  • All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 77 kDa
  • ab109200, at 1/100 dilution, staining Transglutaminase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab109200 staining Transglutaminase 2 in mouse kidney and heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween-20 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS-T + 5% BSA) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.

    See Abreview

Anti-Transglutaminase 2 antibody [EP2957] (ab109200) 使用論文

ab109200 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Mouse Tissue sections (Kidney, Heart)
Specification Kidney, Heart
Permeabilization Yes - 0.1% Tween-20
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Jun 02 2014

Thank you for your enquiry.

I can confirm that ab109200 Transglutaminase 2 antibody [EP2957] has the following alignments to the isoforms of the protein:

Isoform 1 100%
isoform 2 and 3 26%

Therefore, the antibody i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"