Anti-Transglutaminase 2 抗体 [CUB 7402] (ab2386)

製品の概要

  • 製品名
    Anti-Transglutaminase 2 antibody [CUB 7402]
    Transglutaminase 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CUB 7402] to Transglutaminase 2
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rabbit, Guinea pig, Cat, Dog, Human
  • 免疫原

    Purified guinea pig liver Transglutaminase 2.

  • エピトープ
    Epitope mapping for this antibody is amino acid 447-478
  • ポジティブ・コントロール
    • IHC-P: Human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. PubMed: 18397883
ICC/IF Use at an assay dependent concentration. PubMed: 19398782
Flow Cyt 1/10.

Methanol fixed cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • 配列類似性
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

画像

  • ab2386 staining breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 30 minutes at 22°C.

  • ab2386 staining Transglutaminase 2 in human SW480 colorectal carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in 1X PBS/ 1% BSA for 7 min at RT and permeabilized with 0.1% Triton-X100 in PBS for 5 min at RT. Samples were incubated with primary antibody (1/100: in 1X PBS / 1% BSA) for 20 minutes at 20°C. An FITC-conjugated rat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody. Slides counterstained with DAPI, and mounted with commercially available Prolong Slow-fade mounting medium.

    See Abreview

  • Overlay histogram showing HCT116 cells stained with ab2386 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2386, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

  • ab2386 staining Transglutaminase 2 in rat lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein in PBS for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/200 in TBS + BSA) for 20 minutes. ab98782 was used as the secondary antibody at a dilution of 1/400.

    See Abreview

参考文献

This product has been referenced in:
  • Espitia Pinzon N  et al. Astrocyte-derived tissue Transglutaminase affects fibronectin deposition, but not aggregation, during cuprizone-induced demyelination. Sci Rep 7:40995 (2017). WB . Read more (PubMed: 28128219) »
  • Merlino G  et al. Stromal Activation by Tumor Cells: An in Vitro Study in Breast Cancer. Microarrays (Basel) 5:N/A (2016). IHC . Read more (PubMed: 27600076) »

See all 12 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (primary skin keratinicytes)
Gel Running Conditions
Reduced Denaturing (gel any Kd from BioRad)
Loading amount
30 µg
Specification
primary skin keratinicytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Julien Coutier

Verified customer

投稿 May 12 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Lung
Blocking step
Casein in PBS as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 24 2016

Application
Flow Cytometry
Sample
Rhesus monkey Cell (rhesus monkey chorioretinal endothelial cell (RF/6)
Permeabilization
Yes - 0.02% Triton X-­100 with 0.1mg/mL bovine serum albumin (BSA) for 7 min
Gating Strategy
size and granularity
Specification
rhesus monkey chorioretinal endothelial cell (RF/6
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with 0.05% Trypsin-EDTA
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 03 2015

Thank you for contacting us.

This clone has been published in many publications. You may find publications about the specificty of this clone e.g.

http://www.fasebj.org/content/suppl/2007/07/31/fj.06-7598com.DC1/06-7598SupplementalDat...

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Thank you for contacting us.

We have realized that there was an error on the datasheet which has now been corrected. The correct epitope mapping was from 447-478 amino acid instead of 451-454 which is a too small sequence. We apologize for thi...

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Thank you for your email.

The epitope mapping for this antibody is from amino acid 447 to 478. This is about 30 amino acids, which is largely sufficient for an antibody epitope. Please let me know if you still would have some concerns.
<...

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Thank you for contacting us.

The epitope mapping for this antibody is from amino acid 447 to 478.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (Human SH-SY5Y neuroblatoma cells)
Loading amount
10 µg
Specification
Human SH-SY5Y neuroblatoma cells
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mrs. Adelheid Hanes

Verified customer

投稿 Nov 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (primary dermal fibroblasts from mouse)
Specification
primary dermal fibroblasts from mouse
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% (v/v) Triton X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SW480 colorectal carcinoma)
Specification
SW480 colorectal carcinoma
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in PBS
Username

Ms. Doriana Cellura

Verified customer

投稿 Sep 17 2009

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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