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Purified guinea pig liver Transglutaminase 2.
Our Abpromise guarantee covers the use of ab2386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|AP||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method. 10 mM citrate buffer, pH 6.0.|
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19398782|
|Flow Cyt||1/10. (methanol fixed cells)
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Other||Use at an assay dependent concentration.|
ab2386 staining breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 30 minutes at 22°C.
ab2386 staining Transglutaminase 2 in human SW480 colorectal carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in 1X PBS/ 1% BSA for 7 min at RT and permeabilized with 0.1% Triton-X100 in PBS for 5 min at RT. Samples were incubated with primary antibody (1/100: in 1X PBS / 1% BSA) for 20 minutes at 20°C. An FITC-conjugated rat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody. Slides counterstained with DAPI, and mounted with commercially available Prolong Slow-fade mounting medium.