製品の概要

  • 製品名
    Anti-Transglutaminase 2 antibody
    Transglutaminase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Transglutaminase 2
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from center region of human TGM2 conjugated to KLH

  • ポジティブ・コントロール
    • K562 cell lysate Human lung carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50.

ターゲット情報

  • 機能
    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • 配列類似性
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

画像

  • All lanes : Anti-Transglutaminase 2 antibody (ab71099)

    Lane 1 : HUVEC lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 77 kDa
  • Immunofluorescence image of U251 cells labelling Transglutaminase 2 with ab71099. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab71099 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Transglutaminase 2 immunoreactivity is localised to the cytoplasm.

  • Anti-Transglutaminase 2 antibody (ab71099) at 1/60 dilution + K562 cell lysate at 35 µg

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • Formalin-fixed and paraffin-embedded human lung carcinoma tissue labelled with ab71099 at 1/10 diution followed by peroxidase conjugation to the secondary antibody and DAB staining.

参考文献

ab71099 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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